The purpose of the experimental development of winter barley fertilisation consultancy was examine the impact of different N supply levels on the macro- and microelement concentration of winter barley at tillering, as well as its yield on chernozem meadow soil at well separated soil nutrient supply levels in a long-term fertilisation experiment. The long-term fertilisation experiment was established in 1989 on deeply calcareous chernozem meadow soil using 4–4 N, P and K supply levels and a whole treatment combination with 64 treatments and three replications. This study presents the N fertilisation results of experiments performed in 2011 and 2012.

1. In 2011, when the AL-K2O supply of the soil was 320–324 mg kg-1 and the AL-P2O5 supply was 118–251 mg kg-1, the increasing dose of N fertilisation significantly increase the nutrient concentration of winter barley at tillering in the case of N, K, Ca and Mn in comparison with the control treatment. In 2012, significant increase in concentration was observed only in the case of N, Mn and Zn as a result of better N supply.

2. The highest nutrient concentrations resulted from 240 kg ha-1 N supply both in the case of macro- and microelements, but the maximum yield was obtained in the case of 160 kg ha-1 N supply in both examined years.

3. The satisfactory nourishment status of the maximum yield can be characterized with the following nutrient concentrations in the development level of winter barley at tillering: N 3.47–3.85%, P 0.29–0.41%, K 3.07–4.16%, Ca 0.46–0.57%, Mg 0.18–0.22%, Na 0.18–0.23%, Mn 57–74 mg kg-1, Zn 22–30 mg kg-1, Cu 5–7 mg kg-1, B 4–5 mg kg-1, Mo 0.19–0.22 mg kg-1.

4. There was a close correlation between the nutrient concentration of winter barley and grain yield in the case of N, while the correlation was moderate in the case of K and Ca.

Keywords: N supply, winter barley, macro- and microelements, nutrient concentration