Both cold pre-treatment and n-butanol treatment increased the proportion of microspores providing androgenic response. However, the most significant microspore induction which was greatly increased in comparison with the control was induced by simultaneously performing both treatments (20.95 embryos per 100 anthers).
In order to perform an analysis of the effect of treatments on the cellular structure, the microtubules were marked with immunofluorescence and actin filaments were marked with rhodamine phalloidin. Changes in cell structure were tracked with histological methods.
Cold pre-treatment increased the amount of actin filaments in microspores, while there was no impact observed on the microtubule network. On the contrary, the use of n-butanol resulted in the reversible depolymerisation of microtubules, while the amount of actin filaments remained the same. The obtained results support the hypothesis that the transition between the generative development of microspores and the androgenic development path is assisted by the reorganisation of the cell structure, possibly by means of helping symmetrical divisions.
Download (PDF, 94 KB)
Publication Ethics and Malpractice Statement of Növénytermelés: The journal follows the CODE OF CONDUCT AND BEST PRACTICE GUIDELINES FOR JOURNAL EDITORS announced by the Committee on Publication Ethics.
Mezőgazdaság-, Élelmiszertudományi és Környezetgazdálkodási Kar
4032 Debrecen, Böszörményi út 138.
4015 Debrecen, Pf. 36.
(06 52) 508-310