The MALDI-TOF (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry process is suitable for effectively obtaining information on the macromolecules of the test sample, its protein profile, and can be used in several ways for the mass spectrum recorded in the process of obtaining the results. This study aimed at investigating the usability of MALDI-TOF MS for identification purposes. The test seed used in the study was wheat (Triticum aestivum L. cv. Balitus). Five types of extraction buffer (NaCl neutral buffer, NaCl acid buffer, 1-propanol buffer, 2-propanol buffer, 60% ethanolic buffer) were used and we examined which extracting agent yields the best and purest protein and molecular profile.

Of the five tested solutions, 60% ethanol buffer was the most suitable to obtain reliable results. Wheat grains were ground separately and 1 mg was used for the measurements and each seed is placed in a separate Eppendorf tube. The obtained results are relevant and true to reality. Important, reliable measurements make it easy to check seed production and to perform quick homogeneity testing.

Keywords: gluten, MALDI-TOF MS, protein composition, seed, wheat